Nous cherchons constamment à améliorer les protocoles de production des vecteurs (approches moléculaires, cellulaires et virologiques) dans le but :
- d’augmenter la qualité des vecteurs produits,
- de réduire les coûts et les délais de production,
- et d’étendre nos services.
Les adénovirus sont des agents pathogènes courants dans la population humaine. Ils persistent également pendant des décennies. Par conséquent, l'injection de vecteurs Ad chez l'homme peut être compliquée. Nos objectifs de R&D sont de comprendre comment ces vecteurs sont ciblés par des protéines extracellulaires qui peuvent modifier leur immunogénicité dans les tissus humains. Notre objectif est ainsi d'essayer de prédire les effets indésirables qui pourraient survenir lorsque les vecteurs sont injectés dans le cerveau. Cela permettra d’élargir notre place sur la scène internationale en donnant accès à des vecteurs CAV-2 robustes pour le transfert de gène non seulement chez l’animal, mais également chez l’homme. Les projets de R&D font l'objet de collaborations internationales entre le plateau IGMM de la plateforme et des laboratoires implantés aux Pays-Bas, au Royaume-Uni et aux États-Unis.
Dans le cadre des programmes de R&D Biocampus Montpellier, le plateau IGF de la plateforme développe un projet conjoint avec l’équipe de Carole Crozet (Institut des Neurosciences de Montpellier).
Le but de ce projet est de mettre en place une approche permettant d’augmenter l’efficacité d’édition du génome de cellules souches pluripotentes induites humaines (hiPSCs) comparativement aux méthodes utilisées habituellement, tout en diminuant les effets non-spécifiques de ces dernières. L’approche que nous développons actuellement se base sur l’utilisation de nanoparticules dérivées des rétrovirus murins (Mangeot et coll., 2019). Ce type de vectorisation permet de délivrer directement le complexe Cas9/ARN guide (gRNA) dans les cellules. Cette approche sera couplée avec l’utilisation d’un AAV (Adeno-associated virus) pour introduire l’ADN donneur afin d’induire la modification génétique désirée par recombinaison homologue (Martin and coll. 2019 and Chen and coll., 2019).
Publications
En cas de publications de données impliquant l'utilisation de vecteurs produits par les plateaux PVM, nous demandons à nos utilisateurs de nous mentionner dans la section remerciement en citant la phrase suivante "We acknowledge the vector core facility of Montpellier /Plateforme de Vectorologie de Montpellier, BioCampus, CNRS-UM-INSERM, Montpellier, France".
Année | Auteurs | Titre | Journal | DOI | Type |
---|---|---|---|---|---|
2018 | Rozeske, Robert R.; Jercog, Daniel; Karalis, Nikolaos; Chaudun, Fabrice; Khoder, Suzana; Girard, Delphine; Winke, Nânci; Herry, Cyril | Prefrontal-Periaqueductal Gray-Projecting Neurons Mediate Context Fear Discrimination | Neuron | 10.1016/j.neuron.2017.12.044 | indirect |
2018 | Collins, David P.; Anastasiades, Paul G.; Marlin, Joseph J.; Carter, Adam G. | Reciprocal Circuits Linking the Prefrontal Cortex with Dorsal and Ventral Thalamic Nuclei | Neuron | 10.1016/j.neuron.2018.03.024 | indirect |
2018 | Salay, Lindsey D.; Ishiko, Nao; Huberman, Andrew D. | A midline thalamic circuit determines reactions to visual threat | Nature | 10.1038/s41586-018-0078-2 | indirect |
2018 | Li, Shu-Jing; Vaughan, Alexander; Sturgill, James Fitzhugh; Kepecs, Adam | A Viral Receptor Complementation Strategy to Overcome CAV-2 Tropism for Efficient Retrograde Targeting of Neurons | Neuron | 10.1016/j.neuron.2018.05.028 | indirect |
2018 | Giannotti, Giuseppe; Barry, Sarah M.; Siemsen, Ben M.; Peters, Jamie; McGinty, Jacqueline F. | Divergent Prelimbic Cortical Pathways Interact with BDNF to Regulate Cocaine-seeking | The Journal of Neuroscience: The Official Journal of the Society for Neuroscience | 10.1523/JNEUROSCI.1332-18.2018 | indirect |
2018 | Han, Wenfei; Tellez, Luis A.; Perkins, Matthew H.; Perez, Isaac O.; Qu, Taoran; Ferreira, Jozelia; Ferreira, Tatiana L.; Quinn, Daniele; Liu, Zhong-Wu; Gao, Xiao-Bing; Kaelberer, Melanie M.; Bohórquez, Diego V.; Shammah-Lagnado, Sara J.; de Lartigue, Guillaume; de Araujo, Ivan E. | A Neural Circuit for Gut-Induced Reward | Cell | 10.1016/j.cell.2018.08.049 | indirect |
2018 | Fernandez, Sebastian P.; Broussot, Loïc; Marti, Fabio; Contesse, Thomas; Mouska, Xavier; Soiza-Reilly, Mariano; Marie, Hélène; Faure, Philippe; Barik, Jacques | Mesopontine cholinergic inputs to midbrain dopamine neurons drive stress-induced depressive-like behaviors | Nature Communications | 10.1038/s41467-018-06809-7 | indirect |
2018 | Ramanathan, Karthik R.; Jin, Jingji; Giustino, Thomas F.; Payne, Martin R.; Maren, Stephen | Prefrontal projections to the thalamic nucleus reuniens mediate fear extinction | Nature Communications | 10.1038/s41467-018-06970-z | indirect |
2018 | Swift, Kevin M.; Gross, Brooks A.; Frazer, Michelle A.; Bauer, David S.; Clark, Kyle J. D.; Vazey, Elena M.; Aston-Jones, Gary; Li, Yong; Pickering, Anthony E.; Sara, Susan J.; Poe, Gina R. | Abnormal Locus Coeruleus Sleep Activity Alters Sleep Signatures of Memory Consolidation and Impairs Place Cell Stability and Spatial Memory | Current biology: CB | 10.1016/j.cub.2018.09.054 | indirect |
2018 | Vander Weele, Caitlin M.; Siciliano, Cody A.; Matthews, Gillian A.; Namburi, Praneeth; Izadmehr, Ehsan M.; Espinel, Isabella C.; Nieh, Edward H.; Schut, Evelien H. S.; Padilla-Coreano, Nancy; Burgos-Robles, Anthony; Chang, Chia-Jung; Kimchi, Eyal Y.; Beyeler, Anna; Wichmann, Romy; Wildes, Craig P.; Tye, Kay M. | Dopamine enhances signal-to-noise ratio in cortical-brainstem encoding of aversive stimuli | Nature | 10.1038/s41586-018-0682-1 | indirect |
2018 | Barik, Arnab; Thompson, James Hunter; Seltzer, Mathew; Ghitani, Nima; Chesler, Alexander T. | A Brainstem-Spinal Circuit Controlling Nocifensive Behavior | Neuron | 10.1016/j.neuron.2018.10.037 | indirect |
2018 | Huh, Carey Y. L.; Peach, John P.; Bennett, Corbett; Vega, Roxana M.; Hestrin, Shaul | Feature-Specific Organization of Feedback Pathways in Mouse Visual Cortex | Current biology: CB | 10.1016/j.cub.2017.11.056 | indirect |
2018 | Asokan, Meenakshi M.; Williamson, Ross S.; Hancock, Kenneth E.; Polley, Daniel B. | Sensory overamplification in layer 5 auditory corticofugal projection neurons following cochlear nerve synaptic damage | Nature Communications | 10.1038/s41467-018-04852-y | indirect |
2018 | Alcaraz, Fabien; Fresno, Virginie; Marchand, Alain R.; Kremer, Eric J.; Coutureau, Etienne; Wolff, Mathieu | Thalamocortical and corticothalamic pathways differentially contribute to goal-directed behaviors in the rat | eLife | 10.7554/eLife.32517 | direct |
2018 | Mestre-Francés, Nadine; Serratrice, Nicolas; Gennetier, Aurélie; Devau, Gina; Cobo, Sandra; Trouche, Stéphanie G.; Fontès, Pascaline; Zussy, Charleine; De Deurwaerdere, Philippe; Salinas, Sara; Mennechet, Franck Jd; Dusonchet, Julien; Schneider, Bernard L.; Saggio, Isabella; Kalatzis, Vasiliki; Luquin-Piudo, M. Rosario; Verdier, Jean-Michel; Kremer, Eric J. | Exogenous LRRK2G2019S induces parkinsonian-like pathology in a nonhuman primate | JCI insight | 10.1172/jci.insight.98202 | direct |
2019 | Li, Hao; Pullmann, Dominika; Jhou, Thomas C. | Valence-encoding in the lateral habenula arises from the entopeduncular region | eLife | 10.7554/eLife.41223 | indirect |
2019 | Lee, Sangjun; Augustine, Vineet; Zhao, Yuan; Ebisu, Haruka; Ho, Brittany; Kong, Dong; Oka, Yuki | Chemosensory modulation of neural circuits for sodium appetite | Nature | 10.1038/s41586-019-1053-2 | indirect |
2019 | Smedley, Elizabeth B.; DiLeo, Alyssa; Smith, Kyle S. | Circuit directionality for motivation: Lateral accumbens-pallidum, but not pallidum-accumbens, connections regulate motivational attraction to reward cues | Neurobiology of Learning and Memory | 10.1016/j.nlm.2019.05.001 | indirect |
2019 | Phillips, Mary L.; Robinson, Holly Anne; Pozzo-Miller, Lucas | Ventral hippocampal projections to the medial prefrontal cortex regulate social memory | eLife | 10.7554/eLife.44182 | indirect |
2019 | Ferretti, Valentina; Maltese, Federica; Contarini, Gabriella; Nigro, Marco; Bonavia, Alessandra; Huang, Huiping; Gigliucci, Valentina; Morelli, Giovanni; Scheggia, Diego; Managò, Francesca; Castellani, Giulia; Lefevre, Arthur; Cancedda, Laura; Chini, Bice; Grinevich, Valery; Papaleo, Francesco | Oxytocin Signaling in the Central Amygdala Modulates Emotion Discrimination in Mice | Current biology: CB | 10.1016/j.cub.2019.04.070 | indirect |
2019 | Otis, James M.; Zhu, ManHua; Namboodiri, Vijay M. K.; Cook, Cory A.; Kosyk, Oksana; Matan, Ana M.; Ying, Rose; Hashikawa, Yoshiko; Hashikawa, Koichi; Trujillo-Pisanty, Ivan; Guo, Jiami; Ung, Randall L.; Rodriguez-Romaguera, Jose; Anton, E. S.; Stuber, Garret D. | Paraventricular Thalamus Projection Neurons Integrate Cortical and Hypothalamic Signals for Cue-Reward Processing | Neuron | 10.1016/j.neuron.2019.05.018 | indirect |
2019 | Zhou, Zheng; Liu, Xuemei; Chen, Shanping; Zhang, Zhijian; Liu, Yuanming; Montardy, Quentin; Tang, Yongqiang; Wei, Pengfei; Liu, Nan; Li, Lei; Song, Ru; Lai, Juan; He, Xiaobin; Chen, Chen; Bi, Guoqiang; Feng, Guoping; Xu, Fuqiang; Wang, Liping | A VTA GABAergic Neural Circuit Mediates Visually Evoked Innate Defensive Responses | Neuron | 10.1016/j.neuron.2019.05.027 | indirect |
2019 | Nakajima, Miho; Schmitt, L. Ian; Halassa, Michael M. | Prefrontal Cortex Regulates Sensory Filtering through a Basal Ganglia-to-Thalamus Pathway | Neuron | 10.1016/j.neuron.2019.05.026 | indirect |
2019 | Lasbleiz, Christelle; Mestre-Francés, Nadine; Devau, Gina; Luquin, Maria-Rosario; Tenenbaum, Liliane; Kremer, Eric J.; Verdier, Jean-Michel | Combining Gene Transfer and Nonhuman Primates to Better Understand and Treat Parkinson's Disease | Frontiers in Molecular Neuroscience | 10.3389/fnmol.2019.00010 | direct |
2019 | Candelas, Miriam; Reynders, Ana; Arango-Lievano, Margarita; Neumayer, Christoph; Fruquière, Antoine; Demes, Elsa; Hamid, Jawed; Lemmers, Céline; Bernat, Claire; Monteil, Arnaud; Compan, Vincent; Laffray, Sophie; Inquimbert, Perrine; Le Feuvre, Yves; Zamponi, Gerald W.; Moqrich, Aziz; Bourinet, Emmanuel; Méry, Pierre-François | Cav3.2 T-type calcium channels shape electrical firing in mouse Lamina II neurons | Scientific Reports | 10.1038/s41598-019-39703-3 | direct |
2019 | Del Rio, Danila; Beucher, Bertrand; Lavigne, Marina; Wehbi, Amani; Gonzalez Dopeso-Reyes, Iria; Saggio, Isabella; Kremer, Eric J. | CAV-2 Vector Development and Gene Transfer in the Central and Peripheral Nervous Systems | Frontiers in Molecular Neuroscience | 10.3389/fnmol.2019.00071 | direct |
2019 | Wichit, Sineewanlaya; Hamel, Rodolphe; Yainoy, Sakda; Gumpangseth, Nuttamonpat; Panich, Suchawadee; Phuadraksa, Thanawat; Saetear, Phoonthawee; Monteil, Arnaud; Morales Vargas, Ronald; Missé, Dorothée | Interferon-inducible protein (IFI) 16 regulates Chikungunya and Zika virus infection in human skin fibroblasts | EXCLI journal | 10.17179/excli2019-1271 | direct |
2020 | Guo Y, Gabola M, Lattanzio R, Paul C, Pinet V, Tang R, Turali H, Bremond J, Longobardi C, Maurizy C, Da Costa Q, Finetti P, Boissière-Michot F, Rivière B, Lemmers C, Garnier S, Bertucci F, Zlobec I, Chebli K, Tazi J, Azar R, Blanchard JM, Sicinski P, Mamessier E, Lemmers B, Hahne M. | Cyclin A2 maintains colon homeostasis and is a prognostic factor in CRC | J Clin Invest | 10.1172/JCI131517 | direct |
2020 | Olivier Ganier, Malik Lutzmann, Julien Cau, Isabelle Peiffer, Céline Lemmers, Sihem Zitouni, Charles Theillet, Marcel Méchali | Mitosis without DNA replication in mammalian somatic cells | bioRxiv | 10.1101/2020.07.08.193607 | direct |
2020 | di Caudo C, Martínez-Valbuena I, Mundiñano IC, Gennetier A, Hernandez M, Carmona-Abellan M, Marcilla Garcia I, Kremer EJ, Luquin R | CAV-2-Mediated GFP and LRRK2 G2019S Expression in the Macaca fascicularis Brain | Front Mol Neurosci | 10.3389/fnmol.2020.00049 | direct |
2020 | An JJ, Kinney CE, Tan JW, Liao GY, Kremer EJ, Xu B. | TrkB-expressing paraventricular hypothalamic neurons suppress appetite through multiple neurocircuits | Nat Commun | doi: 10.1038/s41467-020-15537-w | direct |
2020 | Hayat H, Regev N, Matosevich N, Sales A, Paredes-Rodriguez E, Krom AJ, Bergman L, Li Y, Lavigne M, Kremer EJ, Yizhar O, Pickering AE, Nir Y. | Locus coeruleus norepinephrine activity mediates sensory-evoked awakenings from sleep | Sci Adv. | 10.1126/sciadv.aaz4232 | direct |
2020 | Martel AC, Elseedy H, Lavigne M, Scapula J, Ghestem A, Kremer EJ, Esclapez M, Apicella P. | Targeted Transgene Expression in Cholinergic Interneurons in the Monkey Striatum Using Canine Adenovirus Serotype 2 Vectors | Front Mol Neurosci | 10.3389/fnmol.2020.00076. | direct |
2020 | Cerpa JC, Marchand AR, Salafranque Y, Pape JR, Kremer EJ, Coutureau E. | Targeting Catecholaminergic Systems in Transgenic Rats With a CAV-2 Vector Harboring a Cre-Dependent DREADD Cassette | Front Mol Neurosci | 10.3389/fnmol.2020.00121 | direct |
2020 | Amani Wehbi , Eric J. Kremer and Iria G. Dopeso-Reyes | Location of the Cell Adhesion Molecule “Coxsackievirus and Adenovirus Receptor” in the Adult Mouse Brain | Front. Neuroanat | 10.3389/fnana.2020.00028 | direct |
2020 | Lavoie A, Liu BH. | Canine Adenovirus 2: A Natural Choice for Brain Circuit Dissection | Front Mol Neurosci | 10.3389/fnmol.2020.00009 | indirect |
2020 | Liu Y, Hegarty S, Winter C, Wang F, He Z. | Viral vectors for neuronal cell type-specific visualization and manipulations. | Curr Opin Neurobiol. | 10.1016/j.conb.2020.03.011 | indirect |
2020 | Gordon-Fennell A, Gordon-Fennell L, Desaivre S, Marinelli M. | The Lateral Preoptic Area and Its Projection to the VTA Regulate VTA Activity and Drive Complex Reward Behaviors | Front Syst Neurosci | 10.3389/fnsys.2020.581830 | indirect |
2020 | Allaway KC, Muñoz W, Tremblay R, Sherer M, Herron J, Rudy B, Machold R, Fishell G | Cellular birthdate predicts laminar and regional cholinergic projection topography in the forebrain | Elife | 10.7554/eLife.63249 | indirect |
2021 | Guo Y, Gabola M, Lattanzio R, Paul C, Pinet V, Tang R, Turali H, Bremond J, Longobardi C, Maurizy C, Da Costa Q, Finetti P, Boissière-Michot F, Rivière B, Lemmers C, Garnier S, Bertucci F, Zlobec I, Chebli K, Tazi J, Azar R, Blanchard JM, Sicinski P, Mamessier E, Lemmers B, Hahne M. | Cyclin A2 maintains colon homeostasis and is a prognostic factor in colorectal cancer | J Clin Invest | 10.1172/JCI131517 | direct |
2021 | Impheng H, Lemmers C, Bouasse M, Legros C, Pakaprot N, Guérineau NC, Lory P, Monteil A | The sodium leak channel NALCN regulates cell excitability of pituitary endocrine cells | FASEB J. | 10.1096/fj.202000841RR | direct |
2021 | Seulgi Kang 1 2, Soyoung Jun 1 2, Soo Ji Baek 1 2, Heeyoun Park 1, Yukio Yamamoto 1, Keiko Tanaka-Yamamoto 1 | Recent Advances in the Understanding of Specific Efferent Pathways Emerging From the Cerebellum | Front Neuroanat | 10.3389/fnana.2021.759948 | indirect |
2021 | Jang Soo Yook,1,† Jihyun Kim,1,2,† and Jinhyun Kim | Convergence Circuit Mapping: Genetic Approaches From Structure to Function | Front Syst Neurosci. | 10.3389/fnsys.2021.688673 | indirect |
2021 | Latoya Stevens 1, Lars Emil Larsen 1 2, Wouter Van Lysebettens 1, Evelien Carrette 1, Paul Boon 1, Robrecht Raedt 1, Kristl Vonck | Optimized Parameters for Transducing the Locus Coeruleus Using Canine Adenovirus Type 2 (CAV2) Vector in Rats for Chemogenetic Modulation Research | Front Neurosci | 10.3389/fnins.2021.663337 | indirect |
2021 | Giulia Massaro 1, Amy F Geard 1 2, Wenfei Liu 1, Oliver Coombe-Tennant 1, Simon N Waddington 2 3, Julien Baruteau 4 5, Paul Gissen 5, Ahad A Rahim | Gene Therapy for Lysosomal Storage Disorders: Ongoing Studies and Clinical Development | Biomolecules | 10.3390/biom11040611 | indirect |
2021 | Victoria Cheung 1, Philip Chung 2, Max Bjorni 3, Varvara A Shvareva 3, Yesenia C Lopez 3, Evan H Feinberg | Virally encoded connectivity transgenic overlay RNA sequencing (VECTORseq) defines projection neurons involved in sensorimotor integration | Cell Rep | 10.1016/j.celrep.2021.110131 | indirect |
2021 | Xiaoxiao Lin, Michelle Amalraj, Crisylle Blanton, Brenda Avila, Todd C. Holmes, Douglas A. Nitz, Xiangmin Xu | Noncanonical projections to the hippocampal CA3 regulate spatial learning and memory by modulating the feedforward hippocampal trisynaptic pathway | Plos Biology | 10.1371/journal.pbio.3001127 | indirect |
2021 | Iria Gonzalez Dopeso-Reyes 1, Mathieu Wolff 2, Melissa R Andrews 3, Eric J Kremer | Editorial: Tropism, Mapping, Modeling, or Therapy Using Canine Adenovirus Type 2 (CAV-2) Vectors in the CNS | Front Mol Neurosci | 10.3389/fnmol.2021.636476 | direct |
2021 | Eric J Kremer | What is the risk of a deadly adenovirus pandemic? | PLoS Pathog | 10.1371/journal.ppat.1009814 | direct |
2021 | Thi Thu Phuong Tran 1 2, Tuan Hiep Tran 1 3 4, Eric J Kremer 1 | IgG-Complexed Adenoviruses Induce Human Plasmacytoid Dendritic Cell Activation and Apoptosis | Viruses | 10.3390/v13091699 | direct |
2021 | Coraline Chéneau 1, Karsten Eichholz 1, Tuan Hiep Tran 1, Thi Thu Phuong Tran 1, Océane Paris 1, Corinne Henriquet 2, Jeffrey J Bajramovic 3, Martine Pugniere 2, Eric J Kremer 1 | Lactoferrin Retargets Human Adenoviruses to TLR4 to Induce an Abortive NLRP3-Associated Pyroptotic Response in Human Phagocytes | Front Immunol | 10.3389/fimmu.2021.685218 | direct |